| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
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| Autores: |
Trangoni,Marcos D.
Gioffré,Andrea K.
Cerón Cucchi,María E.
Caimi,Karina C.
Ruybal,Paula
Zumárraga,Martín J.
Cravero,Silvio L.
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| Data: |
2015-06-01
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| Ano: |
2015
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| Palavras-chave: |
Loop-mediated isothermal amplification
Molecular typing
Brucellosis
Paratuberculosis
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| Resumo: |
In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols were suitable for specifically identifying the respective pathogens; in the case of Brucella, it also allowed the identification of all the biovars tested. We conclude that LAMP is a suitable rapid molecular typing tool that could help to shorten the time required to identify insidious bacteria in low-complexity laboratories, mainly in developing countries.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-838246220131206
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.46 n.2 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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